The Fundamentals of GC
Classroom Based 1 Day Course: Level 1
For the less experienced chromatographer or those wishing to update their skills, this course covers the fundamentally important concepts in modern GC analysis. Basics of the chromatographic process, sample preparation, inlet systems, column and detector selection are important topics covered to give the participant a thorough grounding in the technique. Instrument hardware is also covered with basic troubleshooting and maintenance tips as well as an introduction to chromatographic optimisation.
» Basics of the Chromatography Process
» Sample Preparation Protocols
» Sample Introduction
» Columns and Temperature programming
» Measuring & Optimising Chromatographic Parameters
GC Troubleshooting and Maintenance
Classroom Based 1 Day Course: Level 2
A logical approach to troubleshooting is explored using both the Component (hardware based) and Symptomatic (chromatogram based) perspectives. Best practice for instrument maintenance and column handling as well as routines for cleaning and deactivating inlet and detection systems is discussed to aid the user in prolonging the intervals between essential system maintenance. In-depth treatment of the causes of peak shape and baseline anomalies are fully covered, this course is invaluable to anyone who wishes to gain further insight into the problems associated with GC analysis.
» Approaches to Logical Troubleshooting
» Component Perspective
» Symptomatic Perspective – Baselines
» Symptomatic Perspective – Peaks
» Routine Maintenance and Protocols
Practical GC Troubleshooting and Maintenance
Classroom and Laboratory Based 2 Day Course
As well as the above classroom based content the following laboratory based sessions are included to make an all encompassing 2 day course.
» Column Installation
» Inlet Maintenance
» Inlet and Oven Parameters
» Detector Maintenance
» Developing Troubleshooting Strategy’s
Practical GC Method Development
Classroom and Laboratory Based 2 Day Course: Level
Column dimensions, phase type, inlet type and operating conditions, detector settings and optimisation along with sample preparation regimes are all crucial and often baffling choices available to the chromatographer developing methods for GC analysis. This course takes a logical and progressive approach to the subject and discusses the important ‘make or break’ choices.
Supplemented by a host of real world separation examples, tutorial and practical exercises to aid understanding.
More experienced chromatographers can use this course to cut method development lead times drastically, armed with a logical approach and a wealth of new knowledge on how to make the right decision during each aspect of development.
» Setting Method Development Objectives
» Sample Preparation
» Inlet and Flow Rate Parameters
» Choosing a Column & Temperature
» Optimisation Strategies
» Putting it all together!
Practical Fast GC
Classroom and Laboratory Based 1 Day Course: Level 4
This course is designed as a comprehensive introduction to the principles and practice of Fast Gas Chromatography.
The course is a mix of theory, simulation and lab sessions that allow the student to quickly gain knowledge on the theory, practice and limitations of the common approaches to speeding up GC analysis. The main factors in speeding up analysis – reduced column internal diameter, use of shorter columns, use of hydrogen as a carrier with increased carrier gas linear velocity and the use of low thermal mass and electrically heated columns are discussed in detail and method conversions are attempted practically.
Instrumentation is also taken into account and the various approaches to Fast GC using standard GC equipment as well as the instrument requirements to use very small small i.d. columns and alternatively heated columns are discussed at length.
The overall aim of the training is to ‘enable’ clients to speed up existing separations or to develop novel ‘Fast’ GC separations using existing equipment or to understand how to quickly adapt equipment to take advantage of these enabling technologies.
» Why Fast GC? – separation speed as a limiting factor in analysis
» Resolution / Selectivity / Efficiency (Chemistry and Physics!)
» Broad Choices for Optimising Efficiency in GC
» Optimising Plate Number – different theories and approaches
» So what happened to selectivity?
» Working with Hydrogen at High Flow
» Adapting instruments for use with 100mm i.d. GC Columns
» Translating existing methods for use with Fast GC
» Data capture and analysis requirements
» Developing Fast GC Methods
FOR MORE INFORMATION PLEASE CONTACT firstname.lastname@example.org