Fundamentals of HPLC
Classroom based 1 day course: Level 1

Course Description:
This one-day course introduces the fundamentally important concepts associated with HPLC analysis including hardware basics, modes of analysis, basic troubleshooting, column chemistry, principles of ionisation and more. Suitable as a refresher for the more experienced chemist or as an invaluable introduction to the technique for those with limited experience, this course provides an invaluable insight into HPLC principles and practice.
» Basics of the Chromatographic Process
» Sample Preparation Protocols
» Separation Mode / Retention Mechanisms
» Quantitation
» Injectors and Columns
» Detectors
» Measuring & Optimising Chromatographic Parameters

HPLC Troubleshooting and Maintenance
Classroom Based 1 Day Course: Level 2

Course Description:
A logical approach to HPLC Troubleshooting and Maintenance is fully explored within this one-day course. Commonly encountered problems and best practice are explored for all major system components including Eluent, Pump, Injection system, Column and Detector. Troubleshooting separation chemistry, interrogation of poor peak shape and retention problems are a fundamentally important part of the course, leading to increased awareness of separation chemistry. Practical exercises are fundamental to this course and familiar equipment can be used to carry out a wide range of laboratory-based tasks.
» Approaches to Logical Troubleshooting
» Component Perspective
» Columns
» Symptomatic Perspective – Baselines
» Symptomatic Perspective – Peaks
» Common Maintenance Procedures

Practical HPLC Troubleshooting and Maintenance
Classroom and Laboratory Based 2 Day Course

Course Description:
As well as the above classroom based content the following laboratory based sessions are included to make an all encompassing 2 day course.
» Instrument problems – identifying the issues
» Common Maintenance Schedule Operations
» Investigative Operations
» Hands-on Pump, Auto sampler and Detector Maintenance procedures
» Common Calibration
» Putting it all together

HPLC Method Development
Classroom Based 2 Day Course: Level 3

Course Description:
For the experienced chromatographer, this course provides a step-by-step approach to method development.
The course includes all of the crucial aspects of method development including; Information gathering, Mode of Chromatography, Development of Eluent systems, Column choice, Principles of Ionisation, Optimisation of important Chromatographic Parameters (R(s), a , k’ and N) and Detector choice and optimisation. Each aspect is discussed in detail with relevant examples used to demonstrate theoretical principles and software based exercises are included to give further understanding.
» Setting Method Development Objectives
» Sample Preparation
» System choices
» Choosing a Column & Mobile Phase
» Optimisation Strategies
» Quantitation & System Characterisation

Practical HPLC Method Development
Classroom and Laboratory Based 2 Day Course

Course Description:
As well as the above classroom based content the following laboratory based sessions are included to make an all encompassing 2 day course.
» Effect of Column Parameters
» Developing methods for neutral samples
» Developing a separation for a mixture of neutral, acidic and basic compounds
» Using a scouting gradient

Fast & Ultra Fast HPLC
Classroom and Laboratory Based 1 Day Course: Level 4

Course Description:
This course is designed as a comprehensive introduction to the principles and practice of Fast and Ultra-Fast HPLC.
The course is a mix of theory, computer simulation and lab sessions that allow the student to quickly gain knowledge on the theory, practice and limitations of the common approaches to speeding up HPLC analysis. The main factors in speeding up analysis – reduced column dimension, sub 2mm particles, superficially porous silica all combined with elevated temperature and pressure – are discussed in detail and method conversions are attempted practically.
Instrumentation is also taken into account and the various approaches to Fast HPLC using conventional pressure as well as Ultra High Pressure system hardware and the importance of extra column volume and hysteresis effects are also discussed and demonstrated. The overall aim of the training is to enable clients to speed up existing separations or to develop novel ‘Fast’ HPLC separations.
» Acronyms and Nomenclature
» Why Fast HPLC? – separation speed as a limiting factor in analysis
» Resolution / Selectivity / Efficiency
» Broad Choices for Optimising Efficiency
» Optimising Plate Number
» Effectively measuring plate number / reduced plate heights / Poppe Plots
» So what happened to selectivity?
» Effects of high pressure on Solvent and Column Properties
» The influence of column geometry
» Importance of temperature control
» Temperature and flow rate interdependence
» Instrument requirements
» Simple Software tools for scaling separations
» Transferring & Developing Methods