LC/MS For the Chromatographer
Classroom Based 1 Day Course: Level 1
The Atmospheric Pressure Interface (API) is the core element to the course with the principles of operation, limitations and applicability fully explored. The course covers ion suppression, the use of Electrospray or APCI and MS-MS data acquisition modes. Optimisation of interface and mass filter settings and how to best utilise reduced dimension LC to speed up sample throughput will be discussed. All popular interface types and mass analysing equipment (Quadrupole, TOF, Ion Trap etc.) will be comprehensively covered.
» HPLC Considerations
» Sensitivity and Throughput
» Ion Sources/Ion Production
» Detector Systems
» Tuning & Calibration
Classroom and Laboratory Based 2 Day Course: Level 2
This course is not only designed to bolster your theoretical understanding of LC-MS as a technique, but to give you the practical skills needed to gain maximum benefit from your LC-MS detector. The subject theory is practically reinforced by running a range of specifically designed experiments that highlight the capabilities and limitations of Mass Analysers including Singe Quad, Triple Quad and Ion Trap Instruments using a range of samples. Both theoretical and practical time is devoted to ensuring that interface (LC) methods are sufficiently optimised such that greatest benefit is achieved from your Mass Spectrometric Detector.
» Fundamentals Review
» Mass Analysers
» Mass Accuracy and Resolution
» Practical Considerations of Chromatographic Methods
» Scan and SIM Functions
» In Source CID
» Developing MRM Experiments
LC/MS Spectral Interpretation
Classroom Based 1 Day Course: Level 3
Do you currently use LC-MS in your laboratory applications?
Would you like to benefit from a greater understanding of how to interpret the mass spectra produced by Electrospray Ionisation (ESI) and Atmospheric Pressure Chemical Ionisation (APCI)?
If so then this course will be invaluable to increasing your confidence and understanding of:
» Mass spectral data analysis
» Structural elucidation
» Product ion generation by Collision Induced Dissociation (CID) characterization
» Advantages and limitations of common mass analyser types
Interpretation of low molecular weight compound mass spectra considers the importance of:
» Understanding isotope patterns and their relative signal responses in assigning elemental composition and confirming chemical structure.
» Understanding how common characteristic production fragmentation series are produced through atomic electronic effects.
» A mechanistic understanding of the Inductive (I) and Alpha (a) cleavage.
» How CID fragmentation can be invaluable for sample identification purposes through first principles interpretation and database searching.
» Inclusion of peptide sequencing by tandem mass spectrometry as required.
Interpretation of high molecular weight compound mass spectra considers the importance of:
» When to report monoisotopic mass or average mass.
» The process of deconvolution of an ESI multiply charged ion series to derive the molecular weight of a compound.
» Explanation of the formation of common adduct ions under different solvent and buffer conditions.
» Solution and source compound dimerisation effects.
» The process of data dependent acquisition and modern detector systems.
» A comprehensive set of tutor-led tutorial questions to facilitate the learning process.
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